Abstract
Immunohistochemistry and Western blotting were used to determine the distribution of orexin receptors in the rat brain. Strong orexin receptor 1 (OX1R) immunoreactivity was detected in the hypothalamus including the arcuate, ventromedial, and tuberomammillary nuclei that are involved in feeding regulation. The neuropeptide Y- and proopiomelanocortin-containing neurons of the arcuate nucleus, which act to stimulate or to inhibit feeding, respectively, displayed intense OX1R immunoreactivity by double immunostaining. Western blotting analysis yielded a 50-kDa major band of OX1R.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibody Specificity / immunology
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Appetite Regulation / physiology*
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Arcuate Nucleus of Hypothalamus / cytology
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Arcuate Nucleus of Hypothalamus / metabolism
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Carrier Proteins / metabolism*
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Hypothalamic Area, Lateral / cytology
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Hypothalamic Area, Lateral / metabolism
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Hypothalamus / cytology
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Hypothalamus / metabolism*
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Immunohistochemistry
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Intracellular Signaling Peptides and Proteins*
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Male
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Neurons / cytology
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Neurons / metabolism*
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Neuropeptide Y / metabolism
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Neuropeptides / metabolism*
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Obesity / metabolism*
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Obesity / physiopathology
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Orexin Receptors
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Orexins
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Pro-Opiomelanocortin / metabolism
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Rats
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Rats, Sprague-Dawley
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Receptors, G-Protein-Coupled
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Receptors, Neuropeptide / metabolism*
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Ventromedial Hypothalamic Nucleus / cytology
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Ventromedial Hypothalamic Nucleus / metabolism
Substances
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Carrier Proteins
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Hcrtr1 protein, rat
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Intracellular Signaling Peptides and Proteins
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Neuropeptide Y
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Neuropeptides
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Orexin Receptors
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Orexins
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Receptors, G-Protein-Coupled
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Receptors, Neuropeptide
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Pro-Opiomelanocortin