Abstract
We have previously identified a mAb that binds to a molecule expressed preferentially on the surface of cycling thymocytes. In this study the molecule recognized by this mAb has been identified in the mouse as CD147 (basigin) by expression cloning. We show that CD147 expression correlates with cycling of immature thymocytes even in the absence of TCRbeta selection and that ligation of this molecule on immature fetal thymocytes inhibits their further development into mature T cells.
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Monoclonal / metabolism
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Antigens, CD*
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Antigens, Neoplasm*
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Antigens, Surface*
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Avian Proteins*
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Base Sequence
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Basigin
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Binding Sites, Antibody / genetics
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Blood Proteins*
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Cell Cycle / genetics
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Cell Cycle / immunology
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Cell Differentiation / genetics
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Cell Differentiation / immunology
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Cell Line
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Cloning, Molecular
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Female
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Fetus
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Humans
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Membrane Glycoproteins / biosynthesis
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / immunology
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Membrane Glycoproteins / physiology*
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Membrane Proteins / genetics
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Membrane Proteins / immunology
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Membrane Proteins / metabolism
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Molecular Sequence Data
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Organ Culture Techniques
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Receptors, Antigen, T-Cell, alpha-beta / biosynthesis
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Thymus Gland / cytology
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Thymus Gland / embryology*
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Thymus Gland / immunology*
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Thymus Gland / metabolism
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Tumor Cells, Cultured
Substances
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Antibodies, Monoclonal
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Antigens, CD
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Antigens, Neoplasm
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Antigens, Surface
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Avian Proteins
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BSG protein, human
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Blood Proteins
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Bsg protein, Gallus gallus
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Bsg protein, mouse
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Bsg protein, rat
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Membrane Glycoproteins
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Membrane Proteins
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Receptors, Antigen, T-Cell, alpha-beta
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Basigin