Micro-capillary liquid chromatography Fourier transform ion cyclotron resonance mass spectrometry--a powerful tool for peptide and protein identification

M Ramström; M Palmblad; A Amirkhani; Y O Tsybin; K E Markides; P Håkansson; J Bergquist

Micro-capillary liquid chromatography Fourier transform ion cyclotron resonance mass spectrometry--a powerful tool for peptide and protein identification

Acta Biochim Pol. 2001;48(4):1101-4.

Authors

M Ramström¹, M Palmblad, A Amirkhani, Y O Tsybin, K E Markides, P Håkansson, J Bergquist

Affiliation

¹ Department of Analytical Chemistry, Uppsala University, Sweden.

PMID: 11995972

Abstract

In order to be able to study complex biological samples, a micro-capillary liquid chromatography system was coupled to a 9.4 T Fourier transform ion cyclotron resonance mass spectrometer. The setup was tested on a tryptic digest of bovine serum albumin, which resulted in high sequence coverage (> 92%) of the protein.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Chromatography, Liquid / methods*
Mass Spectrometry / instrumentation*
Mass Spectrometry / methods*
Peptides / chemistry
Proteins / chemistry
Serum Albumin / metabolism
Spectroscopy, Fourier Transform Infrared / methods*

Substances

Peptides
Proteins
Serum Albumin