Hypocretin-1 (orexin-A) levels in human lumbar CSF in different age groups: infants to elderly persons

Sleep. 2002 May 1;25(3):337-9. doi: 10.1093/sleep/25.3.337.

Abstract

Study objectives: Recent CSF and postmortem brain hypocretin measurements in human narcolepsy suggest that hypocretin deficiency is involved in the pathophysiology of the disease. In this study, we measured CSF hypocretin-1 levels in various age ranges from infants to elder people to investigate the age-dependent change of hypocretin concentrations.

Design: CSF hypocretin levels were compared by age groups and gender. ANOVA was used to examine the influences of these two parameters on CSF hypocretin levels.

Setting: University-based sleep and biology laboratory.

Patients or participants: Two hundred seventy two patients were included in this study, with 157 males and 115 females (0-79 years old).

Interventions: CSF samples were obtained by lumber punctures with informed consents.

Measurements and results: Hypocretin-1 levels are not different in respect to gender or age, although our samples constitute a heterogeneous group with various disease conditions. CSF hypocretin-1 levels in infants under 4 months are similar to those in adults.

Conclusions: Early maturation of hypocretin transmission is suggested. No age- or gender-dependent changes in CSF hypocretin is observed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Age Factors
  • Aged
  • Brain Chemistry
  • Carrier Proteins / analysis
  • Carrier Proteins / cerebrospinal fluid*
  • Culture Techniques
  • Female
  • Humans
  • Infant
  • Intracellular Signaling Peptides and Proteins*
  • Male
  • Middle Aged
  • Narcolepsy / cerebrospinal fluid*
  • Neuropeptides / analysis
  • Neuropeptides / cerebrospinal fluid*
  • Neuropeptides / deficiency
  • Orexins
  • Spinal Puncture

Substances

  • Carrier Proteins
  • HCRT protein, human
  • Intracellular Signaling Peptides and Proteins
  • Neuropeptides
  • Orexins