Abstract
HIV-specific CTL functions were analyzed in HIV-infected individuals who did or did not receive antiretroviral therapy (ART). Results showed that gp 160 (env)-stimulated perforin- and granzyme-expressing CTL, as well as perforin and granzyme-specific mRNA, were reduced in treated patients whereas TNFalpha was increased in ART-treated compared to naive individuals. Reduction of perforin and granzyme-expressing cells was not secondary to impaired IFNgamma production. A defect of CTL is observed in ART-treated individuals; this defect is not dependent on impaired Th cell function. These results reinforce the need for immunomodulants to successfully approach therapy of HIV infection.
MeSH terms
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Anti-HIV Agents / pharmacology
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Anti-HIV Agents / therapeutic use*
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CD8-Positive T-Lymphocytes / enzymology
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CD8-Positive T-Lymphocytes / immunology
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Cytoplasmic Granules / enzymology
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Cytotoxicity, Immunologic
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DNA, Complementary / genetics
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Enzyme Induction
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Gene Products, env / pharmacology
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HIV Infections / drug therapy*
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HIV Infections / immunology
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HIV-1 / immunology*
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Humans
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Interferon-gamma / biosynthesis
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Membrane Glycoproteins / analysis
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Perforin
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Pore Forming Cytotoxic Proteins
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RNA, Messenger / analysis
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Serine Endopeptidases / analysis
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T-Lymphocytes, Cytotoxic / enzymology
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T-Lymphocytes, Cytotoxic / immunology*
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Tumor Necrosis Factor-alpha / biosynthesis
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Tumor Necrosis Factor-alpha / genetics
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Viremia / drug therapy
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Viremia / immunology
Substances
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Anti-HIV Agents
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DNA, Complementary
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Gene Products, env
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Membrane Glycoproteins
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Pore Forming Cytotoxic Proteins
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RNA, Messenger
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Tumor Necrosis Factor-alpha
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Perforin
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Interferon-gamma
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Serine Endopeptidases