Identification of a putative vacuolar serine protease gene in the rice blast fungus, Magnaporthe grisea

Satoru Fukiya; Takao Kuge; Tomomi Tanishima; Teruo Sone; Takashi Kamakura; Isamu Yamaguchi; Fusao Tomita

doi:10.1271/bbb.66.663

Identification of a putative vacuolar serine protease gene in the rice blast fungus, Magnaporthe grisea

Biosci Biotechnol Biochem. 2002 Mar;66(3):663-6. doi: 10.1271/bbb.66.663.

Authors

Satoru Fukiya¹, Takao Kuge, Tomomi Tanishima, Teruo Sone, Takashi Kamakura, Isamu Yamaguchi, Fusao Tomita

Affiliation

¹ Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.

PMID: 12005067
DOI: 10.1271/bbb.66.663

Abstract

We identified and cloned a gene designated SPM1, encoding a serine protease from the rice blast fungus Magnaporthe grisea. SPM1 is a single-copy gene, encoding a subtilisin-like serine protease with 536 amino acids. Analyses of the deduced amino acid sequence of SPM1 suggested that SPM1 would be localized in a vacuole, an important organelle in pathogenicity.

MeSH terms

Amino Acid Sequence
Ascomycota / enzymology*
Ascomycota / genetics*
Ascomycota / pathogenicity
Base Sequence
Cloning, Molecular
DNA Primers
Genes, Fungal
Molecular Sequence Data
Oryza
Plant Diseases / microbiology*
Serine Endopeptidases / genetics*
Subcellular Fractions / enzymology
Vacuoles / enzymology*

Substances

DNA Primers
Serine Endopeptidases

Associated data

GENBANK/AB066277
GENBANK/AI068959
GENBANK/AJ251921