Detergent extract from chlamydial elementary bodies used as antigen for enzyme-linked immunosorbent assay

M A Quevedo Diaz; J Melnicáková; J Kazár; J Hoch; S Senfleben; M Lukácová

doi:10.1016/s0732-8893(02)00362-0

Detergent extract from chlamydial elementary bodies used as antigen for enzyme-linked immunosorbent assay

Diagn Microbiol Infect Dis. 2002 Apr;42(4):237-42. doi: 10.1016/s0732-8893(02)00362-0.

Authors

M A Quevedo Diaz¹, J Melnicáková, J Kazár, J Hoch, S Senfleben, M Lukácová

Affiliation

¹ Institute of Virology, Slovak Academy of Sciences, Dúbravská 9, 84245 Bratislava, Slovak Republic.

PMID: 12007440
DOI: 10.1016/s0732-8893(02)00362-0

Abstract

Soluble antigen (SA) from chlamydial elementary bodies (EBs) was extracted with N-lauroylsarcosine. The extracted SA composed of lipopolysaccharide (LPS) and proteins was compared with EBs using an enzyme-linked immunosorbent assay (ELISA). Patient sera from natural chlamydial infections exhibited ELISA mean absorbance (A(492) and A(405/650)) values 2-5 times higher with SA than with EBs, resulting in a better discrimination between positive and negative human sera.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Antibodies, Bacterial / blood*
Antigens, Bacterial / chemistry*
Blotting, Western
Chlamydia Infections / diagnosis*
Chlamydia trachomatis / chemistry
Chlamydia trachomatis / immunology*
Detergents / chemistry
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay / methods*
Female
Humans
Lipopolysaccharides / chemistry*
Male
Middle Aged
Sarcosine / analogs & derivatives*
Sarcosine / chemistry

Substances

Antibodies, Bacterial
Antigens, Bacterial
Detergents
Lipopolysaccharides
sarkosyl
Sarcosine