Abstract
An osmosensing mechanism of Saccharomyces cerevisiae involves a mitogen-activated protein kinase (MAPK) cascade (HOG pathway). This study aimed to investigate the response of the yeast to osmotic stress. A mutant strain, in which the HOG1 gene was disrupted by TRP1, was constructed. A spontaneous mutant, named YJY45, which suppresses the osmosensitive growth phenotype of the hog1 deletion mutant, was selected and showed a secondary phenotype of temperature sensitivity on YPD containing 0.5 M NaCl at 37 degrees C. Our data indicate that the spontaneous mutation in YJY45 mutant was mapped in KSS1, which is one of the MAPK family. The mutation in KSS1 suppresses the osmolarity-hypersensitive phenotype of the hog1 deletion mutation and restores GPD1 induction.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Gene Expression Regulation, Fungal
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Genes, Fungal
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Genetic Complementation Test
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Glycerolphosphate Dehydrogenase / biosynthesis
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Glycerolphosphate Dehydrogenase / genetics
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MAP Kinase Signaling System* / genetics
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Mitogen-Activated Protein Kinases / genetics
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Mitogen-Activated Protein Kinases / physiology*
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Mutagenesis, Insertional
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Osmolar Concentration
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / physiology*
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Saccharomyces cerevisiae Proteins / biosynthesis
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / physiology*
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Sequence Deletion
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Signal Transduction / genetics
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Suppression, Genetic*
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Transcription Factors / physiology
Substances
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Glycerolphosphate Dehydrogenase
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FUS3 protein, S cerevisiae
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HOG1 protein, S cerevisiae
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KSS1 protein, S cerevisiae
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Mitogen-Activated Protein Kinases