Abstract
During a screening procedure for the discovery of a strong gamma-decalactone producer from ricinoleic acid, we observed that the yeast Pichia guilliermondii accumulated transiently 8-hydroxy-3Z,5Z-tetradecadienoic acid 1 during gamma-decalactone biosynthesis in the stationary phase of growth. The structural elucidation of 1 was based on nuclear magnetic resonance, infrared, ultraviolet and gas chromatography-mass spectrometry experiments. The occurrence of 1 is discussed in relation with previously proposed gamma-decalactone biosynthetic pathways.
MeSH terms
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Chromatography, High Pressure Liquid
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Fatty Acids, Unsaturated / isolation & purification*
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Fatty Acids, Unsaturated / metabolism
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Gas Chromatography-Mass Spectrometry
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Hydroxy Acids / isolation & purification*
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Hydroxy Acids / metabolism
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Lactones / metabolism*
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Nuclear Magnetic Resonance, Biomolecular
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Pichia / metabolism*
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Ricinoleic Acids / metabolism*
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Spectrophotometry, Infrared
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Spectrophotometry, Ultraviolet
Substances
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8-hydroxytetradecadienoic acid
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Fatty Acids, Unsaturated
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Hydroxy Acids
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Lactones
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Ricinoleic Acids
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decan-4-olide
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ricinoleic acid