Abstract
Arginine-specific cysteine proteinases (gingipains-R) from periodontopathic Porphyromonas gingivalis cleaved CD14, a bacterial pattern recognition receptor, on human gingival fibroblasts (HGF). Consequently, gingipains-R reduced lipopolysaccharide-induced interleukin-8 production by HGF, indicating that gingipains-R inhibited CD14-dependent HGF activation and are involved in immune evasion by the bacterium in periodontal tissues.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ADP-ribosyl Cyclase*
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Adhesins, Bacterial
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Amino Acid Chloromethyl Ketones / pharmacology
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Antigens, CD*
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Arginine
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CD40 Antigens / biosynthesis
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CD59 Antigens / biosynthesis
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Cell Membrane / metabolism
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Cells, Cultured
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Cysteine Endopeptidases / immunology*
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Cysteine Endopeptidases / pharmacology
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Cysteine Proteinase Inhibitors / pharmacology
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Down-Regulation / immunology*
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Fibroblasts / cytology
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Fibroblasts / drug effects
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Fibroblasts / immunology*
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GPI-Linked Proteins
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Gingipain Cysteine Endopeptidases
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Gingiva / cytology
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Gingiva / immunology*
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Hemagglutinins / immunology*
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Hemagglutinins / pharmacology
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Humans
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Interleukin-8 / biosynthesis*
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Lipopolysaccharide Receptors / biosynthesis
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Lipopolysaccharide Receptors / immunology*
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Lipopolysaccharides / immunology
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Lipopolysaccharides / pharmacology
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Membrane Glycoproteins / biosynthesis
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Porphyromonas gingivalis / enzymology*
Substances
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Adhesins, Bacterial
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Amino Acid Chloromethyl Ketones
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Antigens, CD
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CD40 Antigens
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CD59 Antigens
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Cysteine Proteinase Inhibitors
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GPI-Linked Proteins
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Gingipain Cysteine Endopeptidases
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Hemagglutinins
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Interleukin-8
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Lipopolysaccharide Receptors
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Lipopolysaccharides
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Membrane Glycoproteins
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Arginine
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ADP-ribosyl Cyclase
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ADP-ribosyl cyclase 2
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Cysteine Endopeptidases
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phenylalanyl-prolyl-arginine-chloromethyl ketone