Oviedomycin, an unusual angucyclinone encoded by genes of the oleandomycin-producer Streptomyces antibioticus ATCC11891

Carmen Méndez; Eva Künzel; Fredilyn Lipata; Felipe Lombó; William Cotham; Michael Walla; Daniel W Bearden; Alfredo F Braña; Jose A Salas; Jürgen Rohr

doi:10.1021/np010555n

Oviedomycin, an unusual angucyclinone encoded by genes of the oleandomycin-producer Streptomyces antibioticus ATCC11891

J Nat Prod. 2002 May;65(5):779-82. doi: 10.1021/np010555n.

Authors

Carmen Méndez¹, Eva Künzel, Fredilyn Lipata, Felipe Lombó, William Cotham, Michael Walla, Daniel W Bearden, Alfredo F Braña, Jose A Salas, Jürgen Rohr

Affiliation

¹ Departamento de Biología Funcional e Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Universidad de Oviedo, 33006 Oviedo, Spain.

PMID: 12027768
DOI: 10.1021/np010555n

Abstract

Our investigations on the discovery of novel natural metabolites using type II polyketide synthase gene probes (actI/III) yielded an unusual angucyclinone, oviedomycin (2), when applied to the oleandomycin (1) producer Streptomyces antibioticus ATCC11891. The novel natural product was produced using S. albus R(-)M(-) as a host strain, into which a cosmid containing the oviedomycin gene cluster was transformed. Its structure was elucidated by NMR spectroscopy and mass spectrometry.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Aminoglycosides / chemistry
Aminoglycosides / isolation & purification*
Ethers, Cyclic / chemistry
Ethers, Cyclic / isolation & purification*
Magnetic Resonance Spectroscopy
Mass Spectrometry
Molecular Structure
Multienzyme Complexes / genetics
Multienzyme Complexes / metabolism
Nuclear Magnetic Resonance, Biomolecular
Spectrophotometry, Ultraviolet
Spectroscopy, Fourier Transform Infrared
Streptomyces antibioticus / genetics*
Streptomyces antibioticus / metabolism*

Substances

Aminoglycosides
Ethers, Cyclic
Multienzyme Complexes
oviedomycin