Abstract
We report the increased expression of inhibitory natural killer (NK) cell receptor, CD94/NKG2A, for human leucocyte antigen (HLA) class I on CD8+ T cells in granulocyte colony-stimulating factor-mobilized peripheral blood mononuclear cells (G-PBMC) after mixed lymphocyte culture (MLC). The addition of purified CD14+ cells to CD14-depleted G-PBMC-induced CD94/NKG2A expression in a dose-dependent fashion; however, this enhancing effect was inhibited by the membrane between responder cells and CD14+ cells. Therefore, CD14+ cells in G-PBMC induce CD94/NKG2A expression on CD8+ T cells, which in turn appear to downregulate alloresponses after allogeneic peripheral blood stem cell transplantation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, CD / metabolism*
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CD8-Positive T-Lymphocytes / immunology*
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Cell Division / immunology
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Granulocyte Colony-Stimulating Factor / pharmacology
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Hematopoietic Stem Cell Mobilization
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Humans
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Killer Cells, Natural / immunology
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Lectins, C-Type*
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Lenograstim
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Leukocytes, Mononuclear / immunology*
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Lipopolysaccharide Receptors / analysis*
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Lymphocyte Activation
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Lymphocyte Culture Test, Mixed
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Membrane Glycoproteins / metabolism*
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NK Cell Lectin-Like Receptor Subfamily C
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NK Cell Lectin-Like Receptor Subfamily D
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Receptors, Immunologic / metabolism*
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Receptors, Mitogen / metabolism
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Receptors, Natural Killer Cell
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Recombinant Proteins / pharmacology
Substances
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Antigens, CD
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KLRC1 protein, human
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KLRD1 protein, human
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Lectins, C-Type
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Lipopolysaccharide Receptors
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Membrane Glycoproteins
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NK Cell Lectin-Like Receptor Subfamily C
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NK Cell Lectin-Like Receptor Subfamily D
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Receptors, Immunologic
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Receptors, Mitogen
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Receptors, Natural Killer Cell
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Recombinant Proteins
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Granulocyte Colony-Stimulating Factor
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Lenograstim