Requirement for NusG for transcription antitermination in vivo by the lambda N protein

J Bacteriol. 2002 Jun;184(12):3416-8. doi: 10.1128/JB.184.12.3416-3418.2002.

Abstract

Transcription antitermination by the bacteriophage lambda N protein is stimulated in vitro by the Escherichia coli NusG protein. Earlier work suggested that NusG was not required for N activity in vivo. Here we present evidence that NusG also stimulates N-mediated transcription antitermination in intact cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins / metabolism*
  • Bacteriophage lambda / metabolism*
  • Escherichia coli / virology
  • Escherichia coli Proteins*
  • Peptide Elongation Factors / metabolism*
  • Terminator Regions, Genetic*
  • Transcription Factors / metabolism*
  • Transcription, Genetic*
  • Viral Regulatory and Accessory Proteins / metabolism*

Substances

  • Bacterial Proteins
  • Escherichia coli Proteins
  • N protein, Bacteriophage lambda
  • NusG protein, E coli
  • Peptide Elongation Factors
  • Transcription Factors
  • Viral Regulatory and Accessory Proteins