Abstract
In the present work, we measured survival and the platinum on the genome after treatment of repair-proficient or repair-deficient Escherichia coli strains with trans-[PtCl(2)(E-iminoether)(2)] and compared these results with the effects of "classical" cisplatin. We found that toxicity of antitumor trans-[PtCl(2)(E-iminoether)(2)] in repair-deficient trains was much less than that of cisplatin. This markedly reduced toxicity was not a consequence of the reduced uptake or low levels of DNA binding in the bacteria cells but rather appeared to reflect DNA binding mode of this trans-platinum drug different from that of cisplatin.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents / chemistry
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Antineoplastic Agents / metabolism
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Antineoplastic Agents / pharmacology*
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Cisplatin / metabolism
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Cisplatin / pharmacology*
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DNA Repair
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Escherichia coli / drug effects*
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Escherichia coli / genetics*
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Escherichia coli / metabolism
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Organometallic Compounds / chemistry
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Organometallic Compounds / metabolism
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Organometallic Compounds / pharmacology*
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Organoplatinum Compounds / chemistry
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Organoplatinum Compounds / metabolism
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Organoplatinum Compounds / pharmacology*
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Stereoisomerism
Substances
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Antineoplastic Agents
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Organometallic Compounds
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Organoplatinum Compounds
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dichloroplatinum(E-iminoether)2
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Cisplatin