Abstract
A LightCycler real-time PCR hybridization probe assay was developed for rapid typing of gene variants of the Bordetella pertussis virulence factor pertussis toxin. The assay correctly identified the ptxS1 alleles of all strains tested, comprising 57 Finnish clinical isolates and 2 vaccine strains. The method is simple, reliable, and suitable for large-scale screening of B. pertussis strains.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacterial Typing Techniques
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Base Sequence
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Bordetella pertussis / classification*
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Bordetella pertussis / genetics
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Bordetella pertussis / metabolism
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Fluorescent Dyes*
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Genetic Variation
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Humans
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Molecular Sequence Data
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Nucleic Acid Hybridization / methods*
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Nucleic Acid Probes*
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Pertussis Toxin*
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Pertussis Vaccine / genetics
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Polymerase Chain Reaction / methods*
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Spectrometry, Fluorescence
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Temperature
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Time Factors
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Virulence Factors, Bordetella / genetics*
Substances
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Fluorescent Dyes
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Nucleic Acid Probes
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Pertussis Vaccine
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Virulence Factors, Bordetella
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Pertussis Toxin