Spectrophotometric determination of alendronate in pharmaceutical formulations via complex formation with Fe(III) ions

J Pharm Biomed Anal. 2002 Jun 15;28(6):1215-20. doi: 10.1016/s0731-7085(02)00021-3.

Abstract

The formation of the complex between alendronate, non-chromophoric bisphosphonate drug important for the treatment of a variety of bone diseases, and iron(III) chloride in perchloric acid solution was studied. The stoichiometric ratio of alendronate to Fe(III) ions in the chromophoric complex was determined to be 1:1. The conditional stability constant was logK'(ave)=4.50(SD=0.15), indicating that the Fe(III)-alendronate complex is a complex of medium stability. The optimum conditions for this reaction were ascertained and a spectrophotometric method was developed for the determination of alendronate in the concentration range 8.1-162.5 microg ml(-1), the detection limit being 2 microg ml(-1). The method was validated for the direct determination of alendronate in tablet dosage formulations.

MeSH terms

  • Alendronate / analysis*
  • Alendronate / chemistry
  • Chemistry, Pharmaceutical
  • Iron / chemistry*
  • Spectrophotometry / methods*

Substances

  • Iron
  • Alendronate