Hypertonic sabouraud broth as a simple and powerful test for Candida dubliniensis screening

Diagn Microbiol Infect Dis. 2002 May;43(1):85-6. doi: 10.1016/s0732-8893(02)00368-1.

Abstract

We developed a new screening test for C. dubliniensis based on its inability to grow on Sabouraud dextrose broth with 6.5% NaCl. A total of 266 clinical yeast isolates and 3 reference strains were tested, including 250 C. albicans and 19 C. dubliniensis strains. All C. albicans isolates tested exhibited significant growth on hypertonic Sabouraud broth up to 96 h, while, all C. dubliniensis isolates did not exhibit any visually detectable growth during the same period.

MeSH terms

  • Candida / classification*
  • Candida / drug effects*
  • Candida / growth & development
  • Candida / isolation & purification
  • Candida albicans / classification
  • Candida albicans / drug effects
  • Candida albicans / growth & development
  • Candida albicans / isolation & purification
  • Candidiasis / diagnosis*
  • Candidiasis / microbiology*
  • Cell Division / drug effects
  • Culture Media / chemistry*
  • Culture Media / pharmacology*
  • Glucose
  • Humans
  • Hypertonic Solutions
  • Mycological Typing Techniques / methods*
  • Osmolar Concentration
  • Sensitivity and Specificity
  • Sodium Chloride
  • Species Specificity
  • Time Factors

Substances

  • Culture Media
  • Hypertonic Solutions
  • Sodium Chloride
  • Glucose