LC determination of loratadine and related impurities

J Pharm Biomed Anal. 2002 Jun 20;29(1-2):35-41. doi: 10.1016/s0731-7085(02)00057-2.

Abstract

Loratadine, an antihistamine, could include in its raw material seven impurities that ought to be separated identified and quantified for drug development and quality control. A HPLC method employing a SymmetryShield RP8 column has been developed and validated for loratadine and related compounds measurement, the last ones under the 0.1% level. The mobile phase consisted of methanol-buffer A (65:35, v/v), being buffer A: H(3)PO(4) 10 mM (H(2)O) brought up to pH 7.00 with triethylamine. UV detection was performed at 244 nm. Validation parameters for linearity, accuracy and precision are in agreement with ICH guidelines for all the analytes and that permits to consider the method reliable and suitable for application to long-term stability and purity studies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid / methods*
  • Histamine H1 Antagonists / analysis*
  • Loratadine / analysis*
  • Reproducibility of Results
  • Technology, Pharmaceutical

Substances

  • Histamine H1 Antagonists
  • Loratadine