Abstract
Proteins containing the caspase recruiting domain (CARD) have emerged as critical regulators of different signal transduction pathways, including those controlling apoptosis and activation of necrosis factor (NF)-kappaB transcription factor. TUCAN/CARDINAL is a recently identified CARD-containing protein involved in regulation of caspases and NF-kappaB activation. We find that TUCAN/CARDINAL associates with DRAL, a p53-responsive gene implicated in induction of apoptosis. We also show that, whereas TUCAN/CARDINAL exerts a suppressive effect on NF-kappaB activity, expression of DRAL results in enhancement of NF-kappaB activation. Thus, our observations suggest that DRAL and TUCAN/CARDINAL may participate in a regulatory mechanism that coordinates cellular responses controlled by NF-kappaB transcription factor.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing*
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CARD Signaling Adaptor Proteins
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Cell Line
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HeLa Cells
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Homeodomain Proteins*
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Humans
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LIM-Homeodomain Proteins
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Muscle Proteins / genetics
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Muscle Proteins / metabolism*
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Mutagenesis, Site-Directed
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NF-kappa B / metabolism*
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Neoplasm Proteins / genetics
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Neoplasm Proteins / metabolism*
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Proteins / metabolism
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Signal Transduction*
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TNF Receptor-Associated Factor 2
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TNF Receptor-Associated Factor 6
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Transcription Factors*
Substances
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Adaptor Proteins, Signal Transducing
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CARD Signaling Adaptor Proteins
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CARD8 protein, human
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Carrier Proteins
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FHL2 protein, human
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Homeodomain Proteins
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LIM-Homeodomain Proteins
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Muscle Proteins
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NF-kappa B
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Neoplasm Proteins
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Proteins
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Recombinant Fusion Proteins
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TNF Receptor-Associated Factor 2
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TNF Receptor-Associated Factor 6
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Transcription Factors