Design and synthesis of biotin chain-terminated glycopolymers for surface glycoengineering

J Am Chem Soc. 2002 Jun 26;124(25):7258-9. doi: 10.1021/ja025788v.

Abstract

Biotin chain-terminated glycopolymers were generated by cyanoxyl-mediated free-radical polymerization using a biotin-derivatized arylamine initiator with high conversion (75%) and low polydispersity (1.30). Streptavidin-biotinylated glycopolymer binding was verified by SDS-PAGE gel shift assay and patterned glycocalyx-mimetic surfaces successfully fabricated.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Biopolymers / chemistry*
  • Biotin / analogs & derivatives*
  • Biotin / chemistry
  • Glycosides / chemical synthesis*
  • Glycosides / chemistry
  • Lactose / chemistry
  • Lectins / chemistry
  • Nuclear Magnetic Resonance, Biomolecular
  • Streptavidin / chemistry

Substances

  • Biopolymers
  • Glycosides
  • Lectins
  • Biotin
  • Streptavidin
  • Lactose