Abstract
Somatic embryogenesis is an example of totipotency and is used as a model system for studying embryogenesis. A reproducible tissue culture system was established for the large-scale induction of Arabidopsis somatic embryos. The method allows maintenance of high embryogenic competence over a one-year period. Using this tissue culture system, the expression of embryo-specific genes (ABI3, LEC1, FUS3) was detected in embryogenic cells and somatic embryos. Exogenous application of abscisic acid enhanced the expression of some late-embryogenesis-abundant (LEA) protein genes in somatic embryos. The experiments show that the method can be used to obtain sufficient amounts of embryogenic material for basic molecular analyses.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Abscisic Acid / pharmacology
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Arabidopsis / chemistry
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Arabidopsis / embryology*
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Arabidopsis / genetics
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Arabidopsis Proteins*
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CCAAT-Enhancer-Binding Proteins / genetics
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CCAAT-Enhancer-Binding Proteins / metabolism
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Culture Techniques / methods*
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Gene Expression Regulation, Developmental
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Gene Expression Regulation, Plant
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Immunohistochemistry
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Plant Proteins / drug effects
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Plant Proteins / genetics
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Plant Proteins / metabolism
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Plants, Genetically Modified
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RNA, Plant / metabolism
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Seeds / embryology*
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Seeds / genetics
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Transcription Factors / genetics
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Transcription Factors / metabolism
Substances
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ABI3 protein, Arabidopsis
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Arabidopsis Proteins
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CCAAT-Enhancer-Binding Proteins
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LEC1 protein, Arabidopsis
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Plant Proteins
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RNA, Plant
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Transcription Factors
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late embryogenesis abundant protein, plant
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Abscisic Acid