Abstract
Spiramine T, an atisine-type diterpene alkaloid isolated from the Chinese herbal medicine Spiraea japonica var. acuta (Rosaceae), was shown to have neuroprotective effects on cerebral ischemia-reperfusion injury. In this study, the effects of spiramine T on antioxidant enzymes and nitric oxide production were evaluated in gerbils subjected to global forebrain ischemia (10 min) and reperfusion (5 days). Spiramine T (1.0 and 2.0 mg kg(-1) i.p.) markedly reduced the content of lipid peroxide (LPO), increased the glutathione peroxidase (GSH-PX) activity, and inhibited the increase of nitric oxidase (NOS) activity and nitric oxide production in the cortex during ischemia-reperfusion in gerbils. These results suggested that the neuroprotective effects of spiramine T were related to modulation of endogenous antioxidant enzymatic activities and reduction of the formation of nitric oxide.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antioxidants / pharmacology*
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Brain Ischemia / drug therapy*
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Brain Ischemia / enzymology
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Brain Ischemia / physiopathology
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Catalase / drug effects
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Catalase / metabolism
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Cerebral Cortex / drug effects*
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Cerebral Cortex / enzymology
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Cerebral Cortex / physiopathology
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Drugs, Chinese Herbal / pharmacology
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Female
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Free Radical Scavengers / pharmacology
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Gerbillinae
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Glutathione / metabolism
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Glutathione Peroxidase / drug effects*
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Glutathione Peroxidase / metabolism
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Heterocyclic Compounds, 4 or More Rings / pharmacology*
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Lipid Peroxides / metabolism
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Male
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Malondialdehyde / metabolism
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Neurons / drug effects
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Neurons / enzymology
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Nitric Oxide / biosynthesis*
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Nitric Oxide Synthase / drug effects
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Nitric Oxide Synthase / metabolism
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Oxidative Stress / drug effects
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Oxidative Stress / physiology
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Reperfusion Injury / drug therapy*
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Reperfusion Injury / enzymology
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Reperfusion Injury / physiopathology
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Superoxide Dismutase / drug effects
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Superoxide Dismutase / metabolism
Substances
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Antioxidants
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Drugs, Chinese Herbal
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Free Radical Scavengers
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Heterocyclic Compounds, 4 or More Rings
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Lipid Peroxides
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spiramine T
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Nitric Oxide
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Malondialdehyde
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Catalase
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Glutathione Peroxidase
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Nitric Oxide Synthase
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Superoxide Dismutase
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Glutathione