Fusion protein of Newcastle disease virus is a glycoprotein with antigenicity. The cDNA of fusion gene of NDV strain F48E8 was cloned by RT-PCR, and the sequence of F48E8 was compared with other two virulent strains, Australia-victoria and Italien. Results show that the sequences of N-terminus are very similar, and cysteine residues and the potential glycosylation sites are relatively conserved. However, there are a few differences in the region of signal peptides.