Abstract
Effects of conditioned emotional stimuli (CES), which induce psychological stress, on the expression of cerebral diazepam binding inhibitor (DBI) mRNA in mouse were examined using a communication box. Cerebral DBI mRNA expression significantly increased in a time-dependent manner after the application of CES. The maximal enhancement of DBI mRNA expression was observed 2 days after the application of CES, and this increase faded out over 7 days after the treatment. Flunitrazepam (1 mg/kg), an agonist for central benzodiazepine (BZD) receptors, completely abolished the CES-induced elevation of cerebral DBI contents and its mRNA expressions. These results indicate that cerebral DBI is enhanced by psychological stress, which is regulated by central BZD receptors.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Anxiety Agents / pharmacology
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Anxiety / genetics
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Anxiety / metabolism
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Anxiety / physiopathology
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Cerebral Cortex / drug effects
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Cerebral Cortex / metabolism*
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Conditioning, Psychological / drug effects
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Conditioning, Psychological / physiology
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Diazepam Binding Inhibitor / metabolism*
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Flunitrazepam / pharmacology
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GABA-A Receptor Agonists
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Gene Expression Regulation / drug effects
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Gene Expression Regulation / physiology*
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Male
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Mice
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Neurons / drug effects
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Neurons / metabolism*
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RNA, Messenger / drug effects
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RNA, Messenger / metabolism*
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Receptors, GABA-A / metabolism
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Stress, Physiological / genetics
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Stress, Physiological / metabolism*
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Stress, Physiological / physiopathology
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Stress, Psychological / genetics
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Stress, Psychological / metabolism*
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Stress, Psychological / physiopathology
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Up-Regulation / drug effects
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Up-Regulation / physiology*
Substances
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Anti-Anxiety Agents
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Diazepam Binding Inhibitor
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GABA-A Receptor Agonists
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RNA, Messenger
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Receptors, GABA-A
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Flunitrazepam