Abstract
The Salmonella enterica serovar Typhimurium znuABC genes encoding a high-affinity zinc uptake system and its regulatory zur gene have been cloned. Salmonella serovar Typhimurium zur and znuC knockout mutants have been constructed by marker exchange. The 50% lethal dose of the znuC mutant increased when either orally or intraperitoneally inoculated in BALB/c mice, while virulence of the zur mutant decreased only when mice were intraperitoneally challenged.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Cation Transport Proteins*
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Cell Line
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Cloning, Molecular
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Disease Models, Animal
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Epithelial Cells / microbiology
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Escherichia coli Proteins*
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Female
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Mice
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Mice, Inbred BALB C
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Mutagenesis
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Phenotype
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Salmonella Infections / microbiology*
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Salmonella typhimurium / genetics
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Salmonella typhimurium / pathogenicity*
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Swine
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Virulence
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Zinc / metabolism*
Substances
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Bacterial Proteins
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Carrier Proteins
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Cation Transport Proteins
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DNA-Binding Proteins
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Escherichia coli Proteins
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ZnuC protein, Salmonella enterica
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Zur protein, E coli
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Zinc