Abstract
For the present report, a novel complex class 1 integron, In60, was characterized. Part of this integron includes the bla(CTX-M-9) gene and its downstream nucleotide sequence, which shares 81% and 78% nucleotide identity with those of kluA-1 beta-lactamase and orf3 of K. ascorbata, respectively. Furthermore, a new insertion sequence, IS3000, has been found in In60. PCR analysis indicates that integron In60 is present in 33 of 34 nonclonal enterobacterial isolates carrying the putative beta-lactamase CTX-M-9.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Conserved Sequence
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DNA Transposable Elements
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DNA, Bacterial / genetics*
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Enterobacteriaceae / enzymology
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Enterobacteriaceae / genetics
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Escherichia coli / enzymology
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Escherichia coli / genetics*
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Escherichia coli Proteins*
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Fungal Proteins / genetics*
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Genes, Bacterial / genetics
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Molecular Sequence Data
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Reverse Transcriptase Polymerase Chain Reaction
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Saccharomyces cerevisiae Proteins*
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Transcription Factors*
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beta-Lactamases / genetics*
Substances
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DNA Transposable Elements
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DNA, Bacterial
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Escherichia coli Proteins
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FZF1 protein, S cerevisiae
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Fungal Proteins
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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CTX-M-9 protein, E coli
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beta-Lactamases