Abstract
Using insulin-like growth factor-binding protein-2 (IGFBP-2) transgenic mice (D mice) as a model of elevated IGFBP-2 expression, which is often found in unphysiological conditions, we found association of IGFBP-2 to purified plasma membranes of many organs. To determine whether the RGD (Arg-Gly-Asp) motif of IGFBP-2 mediates cell surface binding in vivo, we mutated the RGD motif of IGFBP-2 into an RGE (Arg-Gly-Glu) sequence and produced transgenic mice (E mice) which express elevated amounts of mutated IGFBP-2. Our data demonstrate that in vivo IGFBP-2 cell surface association is not dependent on the RGD motif and that mutation of this sequence does not alter growth inhibitory effects of IGFBP-2.
MeSH terms
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Amino Acid Motifs / genetics
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Amino Acid Motifs / physiology
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Animals
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Body Weight / genetics
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Body Weight / physiology*
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Cell Membrane / metabolism
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Insulin-Like Growth Factor Binding Protein 2 / blood
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Insulin-Like Growth Factor Binding Protein 2 / genetics
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Insulin-Like Growth Factor Binding Protein 2 / metabolism*
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Insulin-Like Growth Factor I / analysis
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Insulin-Like Growth Factor I / metabolism
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Insulin-Like Growth Factor II / analysis
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Insulin-Like Growth Factor II / metabolism
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Membrane Proteins / blood
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Mice
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Mice, Transgenic / growth & development
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Mice, Transgenic / physiology
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Oligopeptides / genetics
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Oligopeptides / metabolism*
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Organ Size / genetics
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Organ Size / physiology
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Point Mutation
Substances
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Insulin-Like Growth Factor Binding Protein 2
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Membrane Proteins
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Oligopeptides
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Insulin-Like Growth Factor I
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Insulin-Like Growth Factor II
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arginyl-glycyl-aspartic acid