Production of a Mycobacterium avium ssp. paratuberculosis purified protein derivative (PPD) and evaluation of potency in guinea pigs

E M Steadham; B M Martin; C O Thoen

doi:10.1006/biol.2002.0321

Production of a Mycobacterium avium ssp. paratuberculosis purified protein derivative (PPD) and evaluation of potency in guinea pigs

Biologicals. 2002 Jun;30(2):93-5. doi: 10.1006/biol.2002.0321.

Authors

E M Steadham¹, B M Martin, C O Thoen

Affiliation

¹ Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, U.S.A.

PMID: 12127310
DOI: 10.1006/biol.2002.0321

Abstract

A Mycobacterium avium ssp. paratuberculosis purified protein derivative (PPD) was produced and the biologic activity evaluated in sensitized guinea pigs. The PPD when adjusted to a protein concentration of 1mg/ml induced a delayed-type hypersensitivity response comparable to USDA Johnin OT 133-8707.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacterial Proteins* / administration & dosage
Guinea Pigs
Hypersensitivity, Delayed
Mycobacterium avium / immunology*
Mycobacterium avium / metabolism
Paratuberculosis / diagnosis*
Skin / metabolism
Time Factors
Tuberculin / chemistry*

Substances

Bacterial Proteins
Tuberculin