Evidence for impaired lipolysis in abdominally obese men: postprandial study of apolipoprotein B-48- and B-100-containing lipoproteins

Am J Clin Nutr. 2002 Aug;76(2):311-8. doi: 10.1093/ajcn/76.2.311.

Abstract

Background: Abdominal obesity has been associated with postprandial hypertriglyceridemia. The contribution of intestinally and hepatically derived lipoproteins to this exaggerated postprandial lipemic response is not known.

Objective: We examined the associations between body fatness, fat distribution, and postprandial apolipoprotein (apo) B-48 and apo B-100 concentrations measured in triacylglycerol-rich lipoproteins (TRLs).

Design: Dietary fat tolerance was investigated in 50 men aged 28-67 y. The subjects were given a test meal containing 60 g fat/m(2) body surface area and providing 64% of energy from fat, 18% from carbohydrates, and 18% from protein. The meal provided 7524-9196 kJ, depending on body surface area. Blood samples were collected every 2 h over an 8-h period.

Results: The increase in plasma triacylglycerol after the meal resulted from increases in both apo B-48- and apo B-100-containing lipoproteins. The apo B-100 concentration was the strongest contributor (R(2) = 69.6%, P = 0.0001) to postprandial triacylglycerol in total TRLs; the postprandial increase in triacylglycerol was best predicted by the apo B-48 concentration (R(2) = 32.7%, P = 0.0001). Visceral abdominal fat was significantly associated with high postprandial TRL apo B-48 and apo B-100 concentrations (r = 0.30-0.44, P < 0.05). After the meal, the apo B-100 concentration in small TRLs decreased in 12 subjects. These men showed features of the insulin resistance-dyslipidemic syndrome, including more visceral fat (P = 0.07) and an altered fasting metabolic profile.

Conclusion: A lower lipolytic capacity may contribute to the exaggerated and prolonged postprandial lipemia among abdominally obese men.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Apolipoprotein B-100
  • Apolipoprotein B-48
  • Apolipoproteins B / metabolism*
  • Dietary Fats / metabolism*
  • Humans
  • Hypertriglyceridemia / complications
  • Hypertriglyceridemia / metabolism*
  • Lipolysis*
  • Lipoproteins / blood
  • Male
  • Middle Aged
  • Obesity / etiology
  • Obesity / metabolism*
  • Postprandial Period*
  • Regression Analysis

Substances

  • Apolipoprotein B-100
  • Apolipoprotein B-48
  • Apolipoproteins B
  • Dietary Fats
  • Lipoproteins