Abstract
A novel family of C-type lectin-like genes, denoted multidomain free lectin (MDFL), was identified in the freshwater planaria Girardia (Dugesia) tigrina. We cloned several genes that encode proteins comprising a signal peptide and a number of consecutive C-type lectin-like domains (CTLDs) interconnected by short linker stretches. Analyses of genomic organization, CTLD amino acid sequences, and the overall architecture of these proteins indicate that planarian proteins are a separate family of C-type lectin-like proteins. These genes are expressed in specifically differentiated gland cells of planaria and the corresponding proteins are excreted as components of the planarian body surface mucus.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Motifs
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Amino Acid Sequence
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Animals
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Base Sequence
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Binding Sites
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Calcium / metabolism
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Conserved Sequence
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Exons
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Helminth Proteins / chemistry
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Introns
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Lectins, C-Type / chemistry
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Lectins, C-Type / genetics*
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Microtubules / ultrastructure
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Molecular Sequence Data
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Mucus / metabolism
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Phylogeny
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Planarians / genetics*
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Planarians / ultrastructure
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Protein Sorting Signals
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Protein Structure, Tertiary
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Recombinant Proteins / metabolism
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Sequence Homology, Amino Acid
Substances
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Helminth Proteins
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Lectins, C-Type
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Protein Sorting Signals
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Recombinant Proteins
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scarf protein, Dugesia tigrina
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Calcium
Associated data
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GENBANK/AY057969
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GENBANK/AY057970
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GENBANK/AY057971
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GENBANK/AY057972
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GENBANK/AY057973
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GENBANK/AY057974
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GENBANK/AY057975
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GENBANK/AY057976
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GENBANK/AY057977
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GENBANK/AY057978
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GENBANK/AY057979
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GENBANK/AY057980