Abstract
Chemokine receptor transcripts were defined among CD4+ T cells in lymph nodes of mice with type-1 and type-2 inflammation, respectively, elicited by mycobacterial and schistosomal Ag. CXCR3 and CCR6 transcripts were biased to type-1, and CCR4 transcripts increased in type-1 and type-2 populations. CCR3 and CCR5 signals were too weak to establish differences. CCR8 transcripts were not increased among unstimulated populations. Compared to naïve, type-1 and type-2 populations had reduced CCR7 and enhanced CXCR5 transcripts, consistent with a shift to memory cells. Subset depletion revealed that transcript expression was induced among CD44+ memory T cells. Surprisingly, CCR3 transcripts were enriched among CD44lo fractions. Ag stimulation augmented CXCR3, CCR4, and CCR8 but down-regulated CCR6 and CXCR5. CCR4 showed association with IFN-gamma- and IL-4-producing cells, but other receptor transcripts were expressed among IFN-gamma/IL-4 negative memory T cells. These studies provide several novel findings regarding Th cell chemokine receptor expression in vivo.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigens, Bacterial / immunology*
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Antigens, Protozoan / immunology*
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Female
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Gene Expression Regulation*
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Immunologic Memory
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Interferon-gamma / biosynthesis
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Interferon-gamma / genetics
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Interleukin-4 / biosynthesis
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Interleukin-4 / genetics
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Mice
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Mice, Inbred CBA
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Mycobacterium tuberculosis / immunology*
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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Receptors, CCR3
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Receptors, CCR4
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Receptors, CCR6
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Receptors, CCR7
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Receptors, CCR8
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Receptors, CXCR3
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Receptors, CXCR5
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Receptors, Chemokine / biosynthesis*
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Receptors, Chemokine / genetics
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Receptors, Cytokine / biosynthesis
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Receptors, Cytokine / genetics
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Schistosoma mansoni / immunology*
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T-Lymphocyte Subsets / immunology
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T-Lymphocyte Subsets / metabolism*
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Th1 Cells / immunology
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Th1 Cells / metabolism*
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Th2 Cells / immunology
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Th2 Cells / metabolism*
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Tuberculin / immunology*
Substances
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Antigens, Bacterial
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Antigens, Protozoan
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CCR3 protein, human
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CCR4 protein, human
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CCR6 protein, human
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CCR7 protein, human
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CCR8 protein, human
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CXCR3 protein, human
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CXCR5 protein, mouse
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Ccr3 protein, mouse
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Ccr4 protein, mouse
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Ccr7 protein, mouse
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Cxcr3 protein, mouse
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RNA, Messenger
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Receptors, CCR3
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Receptors, CCR4
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Receptors, CCR6
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Receptors, CCR7
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Receptors, CCR8
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Receptors, CXCR3
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Receptors, CXCR5
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Receptors, Chemokine
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Receptors, Cytokine
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Tuberculin
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Interleukin-4
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Interferon-gamma