[Properties of endoribonuclease activity of proteasomes from K562 cells. III. The specific endonuclease activity of ribonucleoprotein particles (alpha-RNP) tightly bound to chromatin and containing 20S proteosomes]

A G Mittenberg; V A Kulichkova; L N Gauze; I M Konstantinova

[Properties of endoribonuclease activity of proteasomes from K562 cells. III. The specific endonuclease activity of ribonucleoprotein particles (alpha-RNP) tightly bound to chromatin and containing 20S proteosomes]

Tsitologiia. 2002;44(4):364-8.

[Article in Russian]

Authors

A G Mittenberg¹, V A Kulichkova, L N Gauze, I M Konstantinova

Affiliation

¹ Institute of Cytology RAS, St. Petersburg. [email protected]

PMID: 12149780

Abstract

The present work demonstrates the ability of 20S proteasome-containing, tightly bound to chromatin RNP-particles (alpha-RNP) to endonucleolyse specific messenger RNAs (in particular, human mRNA for p53 gene and mRNA for luciferase from Renilla sp.). The dependence of individual mRNA endonucleolysis by alpha-RNP particles on both the substrate and enzyme was found.

Publication types

English Abstract

MeSH terms

Chromatin / metabolism
Cysteine Endopeptidases / analysis
Cysteine Endopeptidases / metabolism*
Endoribonucleases / metabolism*
Genes, p53
Humans
K562 Cells / enzymology*
Luciferases / genetics
Multienzyme Complexes / analysis
Multienzyme Complexes / metabolism*
Proteasome Endopeptidase Complex
RNA, Messenger / metabolism
Ribonucleoproteins, Small Cytoplasmic / chemistry
Ribonucleoproteins, Small Cytoplasmic / metabolism*

Substances

Chromatin
Multienzyme Complexes
RNA, Messenger
Ribonucleoproteins, Small Cytoplasmic
Luciferases
Endoribonucleases
Cysteine Endopeptidases
Proteasome Endopeptidase Complex