Abstract
A gene (neg1) encoding an endo-1,6-beta-D-glucanase from Neurospora crassa was cloned. The putative neg1 was 1443-bp long and encoded a mature endo-1,6-beta-D-glucanase protein of 463 amino acids and signal peptide of 17 amino acids. The purified recombinant protein (Neg1) obtained from Escherichia coli showed 1,6-beta-D-glucanase activity. No genes similar in sequence were found in yeasts and fungi.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular
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Escherichia coli / genetics
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Glycoside Hydrolases / chemistry
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Glycoside Hydrolases / genetics*
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Molecular Sequence Data
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Neurospora crassa / enzymology*
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Neurospora crassa / genetics*
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Open Reading Frames / genetics
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Protein Sorting Signals
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Recombinant Proteins / genetics
Substances
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Protein Sorting Signals
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Recombinant Proteins
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Glycoside Hydrolases