Structural constraints from proton-mediated rare-spin correlation spectroscopy in rotating solids

J Am Chem Soc. 2002 Aug 21;124(33):9704-5. doi: 10.1021/ja026691b.

Abstract

A novel solid-state NMR concept is introduced that allows for the detection of through-space proton-proton contacts in high spectral resolution. The proposed method not only can be utilized to infer sequential assignments and backbone or side chain conformation in solid-phase polypeptides, but it also provides information about the three-dimensional arrangement of the molecule of interest. As a result, the molecular structure can be studied without additional restrictions regarding sample labeling or magic angle spinning rates.

MeSH terms

  • Carbon Isotopes
  • Nitrogen Isotopes
  • Nuclear Magnetic Resonance, Biomolecular / methods*
  • Protons
  • Ubiquitin / chemistry*

Substances

  • Carbon Isotopes
  • Nitrogen Isotopes
  • Protons
  • Ubiquitin