Frequency data of short tandem repeats loci included in the SGM Plus kit and on two pentanucleotide STR loci: Penta E and Penta D [Profiles DNA 2 (1998) 2] included in the PowerPlex16 kit were collected from a sample of 400 (for SGM Plus) and 91 (for Penta E and Penta D) random, unrelated individuals born in the South Poland region.