Challenges in applying microarrays to environmental studies

Jizhong Zhou; Dorothea K Thompson

doi:10.1016/s0958-1669(02)00319-1

Challenges in applying microarrays to environmental studies

Curr Opin Biotechnol. 2002 Jun;13(3):204-7. doi: 10.1016/s0958-1669(02)00319-1.

Authors

Jizhong Zhou¹, Dorothea K Thompson

Affiliation

¹ Environmental Sciences Division, Oak Ridge National Laboratory, PO Box 2008, Oak Ridge, TN 37831, USA. [email protected]

PMID: 12180093
DOI: 10.1016/s0958-1669(02)00319-1

Abstract

Although DNA microarray technology has been used successfully to analyze global gene expression in pure cultures, it has not been rigorously tested and evaluated within the context of complex environmental samples. Adapting microarray hybridization for use in environmental studies faces several challenges associated with specificity, sensitivity and quantitation.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Environmental Microbiology*
Environmental Monitoring / instrumentation*
Environmental Monitoring / methods*
Oligonucleotide Array Sequence Analysis* / trends
Quality Control
Reproducibility of Results
Sensitivity and Specificity