Abstract
Relapses in non-Hodgkin's lymphomas (NHL) could be due to the reappearance of the initial neoplasm or new primary tumours. Discrimination between the two events would allow a more targeted therapeutic approach. VDJ rearrangement was used as marker of clonality in metachronous biopsy specimens from 10 patients with relapsed B-NHL. Complimentary determining region 3 was amplified and sequenced. D-JH was identical in eight matched primary/secondary tumours, confirming the diagnosis of recurrence. In contrast, primary and secondary tumours in two patients were of different clonal origin. Our data indicate that VDJ analysis is a fundamental tool for identification of relapses in NHL.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Aged
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Aged, 80 and over
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Base Sequence
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Complementarity Determining Regions / genetics
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DNA, Neoplasm / genetics
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Diagnosis, Differential
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Female
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Follow-Up Studies
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Gene Rearrangement, B-Lymphocyte, Heavy Chain*
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Humans
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Immunoglobulin Joining Region / genetics
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Immunoglobulin Variable Region / genetics
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Lymphoma, B-Cell / diagnosis*
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Lymphoma, B-Cell / genetics
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Male
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Middle Aged
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Molecular Sequence Data
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Neoplasms, Second Primary / diagnosis*
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Neoplasms, Second Primary / genetics
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Neoplastic Stem Cells / pathology
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Recurrence
Substances
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Complementarity Determining Regions
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DNA, Neoplasm
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Immunoglobulin Joining Region
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Immunoglobulin Variable Region