Abstract
In 2000, at the Université d'Auvergne teaching hospital in Clermont-Ferrand, France, 44 (6.2%) strains of Pseudomonas aeruginosa were found to be resistant to ceftazidime. After genotyping, 34 strains were selected. Nine had an additional beta-lactamase: OXA-21 (n = 6), PSE-1 (CARB-2) (n = 2), or PER-1 (n = 1). Ceftazidime resistance was related solely to the overproduction of the cephalosporinase in 30 strains. Sequencing of five bla(AmpC) genes encoding cephalosporinases with different pIs showed 99% identity with the ampC gene of P. aeruginosa PAO1.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Ceftazidime / pharmacology*
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Ceftazidime / therapeutic use
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Cephalosporinase / genetics
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Cephalosporinase / metabolism
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Cephalosporins / pharmacology*
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Cephalosporins / therapeutic use
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Data Collection
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Drug Resistance, Microbial
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France / epidemiology
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Genes, Bacterial
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Genotype
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Humans
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Microbial Sensitivity Tests
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Prospective Studies
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Pseudomonas Infections / epidemiology*
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Pseudomonas Infections / microbiology*
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Pseudomonas aeruginosa / drug effects*
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Pseudomonas aeruginosa / enzymology*
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beta-Lactamase Inhibitors
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beta-Lactamases / genetics
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beta-Lactamases / metabolism*
Substances
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Cephalosporins
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beta-Lactamase Inhibitors
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Ceftazidime
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Cephalosporinase
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beta-Lactamases