Fission yeast Cdc23 interactions with DNA replication initiation proteins

Curr Genet. 2002 Aug;41(5):342-8. doi: 10.1007/s00294-002-0316-9. Epub 2002 Jul 23.

Abstract

Schizosaccharomyces pombe Cdc23 is an essential DNA replication protein, conserved in eukaryotes and functionally homologous with Saccharomyces cerevisiae Dna43 (Mcm10). We sought evidence for interactions between Cdc23 and the MCM2-7 complex, a component of both the pre-replicative complex and the replication fork. Cdc23 shows genetic interactions with four MCM subunits: cdc23-M36 and cdc23-1E2 alleles both show synthetic phenotypes with mcm2 (cdc19-P1) and mcm6 (mis5-268), and cdc23-M36 is synthetically lethal with mcm4 (cdc21-K46) and with mcm5 (nda4-108). The wild-type cdc23 gene on multicopy plasmids can partially suppress temperature-dependent defects in mcm5 (nda4-108). Two-hybrid analysis demonstrates interactions at the protein-protein level between Cdc23 and Mcm4, Mcm5 and Mcm6. Cdc23 also interacts with four subunits of the Schizosaccharomyces pombe origin recognition complex (ORC) in yeast two-hybrid assay: Orc1, Orc2, Orc5 and Orc6. We found no evidence for interaction between Cdc23 and the MCM recruitment factor Cdc18 (the homologue of Saccharomyces cerevisiae Cdc6). Unlike Cdc18, Cdc23 mRNA shows no significant fluctuation in level through the cell cycle. These data suggest that fission yeast Cdc23 is an MCM-associated factor which has a role in the initiation of DNA replication.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaphase-Promoting Complex-Cyclosome
  • Apc8 Subunit, Anaphase-Promoting Complex-Cyclosome
  • Cell Cycle Proteins / genetics*
  • Cell Cycle Proteins / metabolism
  • DNA Replication*
  • DNA-Binding Proteins / metabolism
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Origin Recognition Complex
  • Saccharomyces cerevisiae Proteins*
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces / metabolism
  • Transcription Factors / metabolism*
  • Two-Hybrid System Techniques
  • Ubiquitin-Protein Ligase Complexes

Substances

  • Apc8 Subunit, Anaphase-Promoting Complex-Cyclosome
  • CDC23 protein, S cerevisiae
  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • Fungal Proteins
  • Origin Recognition Complex
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Ubiquitin-Protein Ligase Complexes
  • Anaphase-Promoting Complex-Cyclosome