Abstract
An endothelial cell-tropic and leukotropic human cytomegalovirus (HCMV) clinical isolate was cloned as a fusion-inducing factor X-bacterial artificial chromosome in Escherichia coli, and the ribonucleotide reductase homolog UL45 was deleted. Reconstituted virus RVFIX and RV Delta UL45 grew equally well in human fibroblasts and human endothelial cells. Thus, UL45 is dispensable for growth of HCMV in both cell types.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Apoptosis
-
Cell Line
-
Chromosomes, Artificial, Bacterial
-
Cloning, Molecular
-
Cytomegalovirus / enzymology
-
Cytomegalovirus / growth & development*
-
Cytomegalovirus Infections / virology
-
Endothelium, Vascular / cytology
-
Endothelium, Vascular / virology*
-
Escherichia coli / genetics
-
Female
-
Fibroblasts / virology
-
Flow Cytometry
-
Gene Deletion
-
Humans
-
Pregnancy
-
Pregnancy Complications, Infectious / virology
-
Ribonucleotide Reductases / chemistry*
-
Ribonucleotide Reductases / genetics
-
Ribonucleotide Reductases / metabolism*
-
Viral Proteins / chemistry*
-
Viral Proteins / genetics
Substances
-
UL45 protein, herpes simplex virus type 1
-
Viral Proteins
-
Ribonucleotide Reductases