Abstract
Vasoactive intestinal contractor (VIC)/endothelin-2 (ET2) is a vasoactive peptide hormone comprising 21 amino acids. The complete nucleotide sequence of the full-length gene encoding preproVIC (PPVIC) was determined. The PPVIC gene contains five exons that span 6 kb and shows a duplication on exons 2 and 3, coding for the VIC and VIC-like peptides respectively. Similarities between the genomic organization of the PPVIC/preproET2 and preproendothelin-1 genes suggest that the two are distantly related. PPVIC gene expression was observed in foetal and adult mouse intestine. The expression level in adults was approx. 10-fold higher than in the foetus, suggesting an involvement of VIC in intestinal development.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cloning, Molecular
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Endothelin-1
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Endothelins / genetics*
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Epithelial Cells / metabolism
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Exons
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Gene Expression
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Humans
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Intercellular Signaling Peptides and Proteins
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Intestinal Mucosa / metabolism*
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Intestines / embryology*
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Mesoderm / metabolism
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Mice
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Molecular Sequence Data
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Muscle, Smooth / metabolism
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Peptides / genetics*
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Peptides / metabolism
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Protein Precursors / genetics*
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Alignment
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Sequence Analysis, DNA
Substances
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Endothelin-1
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Endothelins
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Intercellular Signaling Peptides and Proteins
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Peptides
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Protein Precursors
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vasoactive intestinal constrictor