Abstract
The hematopoietic transcription factor GATA-1 regulates erythropoiesis and beta-globin expression. Although consensus GATA-1 binding sites exist throughout the murine beta-globin locus, we found that GATA-1 discriminates among these sites in vivo. Conditional expression of GATA-1 in GATA-1-null cells recapitulated the occupancy pattern. GATA-1 induced RNA polymerase II (pol II) recruitment to subregions of the locus control region and to the beta-globin promoters. The hematopoietic factor NF-E2 cooperated with GATA-1 to recruit pol II to the promoters. We propose that only when GATA-1 attracts pol II to the locus control region can pol II access the promoter in a NF-E2-dependent manner.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Bone Marrow / metabolism*
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Chromatin / metabolism*
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DNA-Binding Proteins / metabolism*
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Erythroid-Specific DNA-Binding Factors
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GATA1 Transcription Factor
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Globins / genetics
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Mice
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NF-E2 Transcription Factor
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NF-E2 Transcription Factor, p45 Subunit
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Promoter Regions, Genetic
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RNA Polymerase II / metabolism*
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Transcription Factors / metabolism*
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Tumor Cells, Cultured
Substances
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Chromatin
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DNA-Binding Proteins
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Erythroid-Specific DNA-Binding Factors
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GATA1 Transcription Factor
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Gata1 protein, mouse
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NF-E2 Transcription Factor
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NF-E2 Transcription Factor, p45 Subunit
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Nfe2 protein, mouse
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Transcription Factors
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Globins
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RNA Polymerase II