Distinct modes of signal recognition particle interaction with the ribosome

Science. 2002 Aug 23;297(5585):1345-8. doi: 10.1126/science.1072366.

Abstract

Signal recognition particle (SRP), together with its receptor (SR), mediates the targeting of ribosome-nascent chain complexes to the endoplasmic reticulum. Using protein cross-linking, we detected distinct modes in the binding of SRP to the ribosome. During signal peptide recognition, SRP54 is positioned at the exit site close to ribosomal proteins L23a and L35. When SRP54 contacts SR, SRP54 is rearranged such that it is no longer close to L23a. This repositioning may allow the translocon to dock with the ribosome, leading to insertion of the signal peptide into the translocation channel.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Centrifugation, Density Gradient
  • Cross-Linking Reagents
  • Dogs
  • Guanosine Diphosphate / metabolism
  • Guanosine Diphosphate / pharmacology
  • Guanosine Triphosphate / metabolism
  • Guanylyl Imidodiphosphate / metabolism
  • Guanylyl Imidodiphosphate / pharmacology
  • Models, Molecular
  • Plant Proteins / chemistry
  • Plant Proteins / metabolism
  • Precipitin Tests
  • Prolactin / genetics
  • Prolactin / metabolism
  • Protein Binding
  • Protein Precursors / genetics
  • Protein Precursors / metabolism
  • Protein Sorting Signals
  • Protein Transport
  • Receptors, Cytoplasmic and Nuclear / chemistry
  • Receptors, Cytoplasmic and Nuclear / metabolism
  • Receptors, Peptide / chemistry
  • Receptors, Peptide / metabolism
  • Ribosomal Proteins / chemistry
  • Ribosomal Proteins / metabolism*
  • Ribosomes / metabolism*
  • Saccharomyces cerevisiae Proteins*
  • Signal Recognition Particle / chemistry
  • Signal Recognition Particle / metabolism*
  • Succinimides

Substances

  • Cross-Linking Reagents
  • Plant Proteins
  • Protein Precursors
  • Protein Sorting Signals
  • RPL35A protein, S cerevisiae
  • Receptors, Cytoplasmic and Nuclear
  • Receptors, Peptide
  • Ribosomal Proteins
  • SRP54 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Signal Recognition Particle
  • Succinimides
  • signal peptide receptor
  • Guanosine Diphosphate
  • Guanylyl Imidodiphosphate
  • preprolactin
  • Guanosine Triphosphate
  • Prolactin
  • disuccinimidyl suberate