Abstract
Plant dynamin-like proteins consist of a group of high molecular weight GTPase with diverse structural arrangements and cellular localizations. In addition, unlike animal dynamins, there was no evidence for the involvement of any plant dynamin-like protein in clathrin-mediated vesicle trafficking. In this study we demonstrate that ADL6 (Arabidopsis dynamin-like protein 6), due to its domain arrangement, behaves similarly to the animal dynamins. The association of ADL6 with clathrin-coated vesicles was demonstrated by co-fractionation and immunocytochemical studies. ADL6 also interacted via its C-terminus with gamma-adaptin, an adaptor protein of clathrin-coated vesicles. Our results suggest that ADL6 participates in clathrin-mediated vesicle trafficking originating from the Golgi. In addition, our studies demonstrate that ADL6 intrinsic GTPase activity is regulated by its association with acidic phospholipids and an SH3 (Src homology 3)-containing protein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Protein Complex gamma Subunits / genetics
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Adaptor Protein Complex gamma Subunits / metabolism
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Arabidopsis / enzymology
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Arabidopsis / genetics*
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism
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Cation Transport Proteins*
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Clathrin / genetics
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Clathrin / metabolism
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Dynamins / genetics
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Dynamins / metabolism*
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Enzyme Activation / drug effects
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Gene Expression Regulation, Enzymologic
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Gene Expression Regulation, Plant
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Phosphatidic Acids / pharmacology
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Phosphoric Monoester Hydrolases / metabolism
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Protein Binding
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Sodium-Hydrogen Exchangers / genetics
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Sodium-Hydrogen Exchangers / metabolism
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Two-Hybrid System Techniques
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Yeasts / genetics
Substances
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Adaptor Protein Complex gamma Subunits
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Arabidopsis Proteins
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Cation Transport Proteins
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Clathrin
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NHX1 protein, Arabidopsis
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Phosphatidic Acids
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Recombinant Fusion Proteins
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Sodium-Hydrogen Exchangers
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proline-rich protein, Arabidopsis
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Phosphoric Monoester Hydrolases
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ADL6 protein, Arabidopsis
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Dynamins