Abstract
Deleted in malignant brain tumors 1 is a putative tumor suppressor protein in brain, lung, esophageal,gastric, and colorectal cancer. Here we report the mass spectrometric identification of a 3335 Da peptide, which was found in serum-free conditioned medium from 5 of 15 pancreatic adenocarcinoma cell lines but not from 35 carcinoma cell lines and 2 nonmalignant pancreatic duct cell lines. The peptide was the 29 COOH-terminal amino acids from deleted in malignant brain tumors 1. It is suggested that the peptide is generated inside the cells by limited proteolysis and extracellularly secreted. Our peptidomics-based approach will help screen candidate marker peptides for a particular type of cancer.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adenocarcinoma / chemistry
-
Adenocarcinoma / metabolism*
-
Agglutinins*
-
Amino Acid Sequence
-
Calcium-Binding Proteins
-
Culture Media, Serum-Free
-
DNA-Binding Proteins
-
Humans
-
Molecular Sequence Data
-
Pancreatic Neoplasms / chemistry
-
Pancreatic Neoplasms / metabolism*
-
Peptide Fragments / biosynthesis
-
Peptide Fragments / chemistry
-
Peptide Fragments / metabolism
-
Peptide Library
-
Receptors, Cell Surface / biosynthesis*
-
Receptors, Cell Surface / chemistry
-
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
-
Tumor Cells, Cultured
-
Tumor Suppressor Proteins
Substances
-
Agglutinins
-
Calcium-Binding Proteins
-
Culture Media, Serum-Free
-
DMBT1 protein, human
-
DNA-Binding Proteins
-
Peptide Fragments
-
Peptide Library
-
Receptors, Cell Surface
-
Tumor Suppressor Proteins