Abstract
A gene coding a novel isoform of DEAD-box protein named testicular DEAD-box protein (tDbp), presumably involved in testicular function, was identified and characterized. Testicular DEAD-box protein was cloned from a human testis cDNA library. The cDNA microarray hybridization showed that it was expressed at a higher level in adult testis than in embryo testis. Reverse transcription-polymerase chain reaction indicated that tDbp was specifically expressed in testis, but not in some other tissues.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular
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DEAD-box RNA Helicases
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DNA, Complementary / genetics
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Gene Expression
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Gene Library
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Humans
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Male
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Molecular Sequence Data
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Nuclear Proteins / chemistry
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Nuclear Proteins / genetics*
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Nucleocytoplasmic Transport Proteins
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Oligonucleotide Array Sequence Analysis
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Organ Specificity
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Protein Isoforms / chemistry
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Protein Isoforms / genetics*
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RNA Helicases / chemistry
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RNA Helicases / genetics*
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RNA, Messenger / analysis
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Reverse Transcriptase Polymerase Chain Reaction
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Testis / chemistry*
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Testis / embryology
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Testis / growth & development
Substances
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DNA, Complementary
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Nuclear Proteins
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Nucleocytoplasmic Transport Proteins
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Protein Isoforms
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RNA, Messenger
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DDX19B protein, human
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DEAD-box RNA Helicases
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RNA Helicases