Further modification of the Hodge test to screen AmpC beta-lactamase (CMY-1)-producing strains of Escherichia coli and Klebsiella pneumoniae

Dongeun Yong; Rojin Park; Jong Hwa Yum; Kyungwon Lee; Eung Chil Choi; Yunsop Chong

doi:10.1016/s0167-7012(02)00053-2

Further modification of the Hodge test to screen AmpC beta-lactamase (CMY-1)-producing strains of Escherichia coli and Klebsiella pneumoniae

J Microbiol Methods. 2002 Nov;51(3):407-10. doi: 10.1016/s0167-7012(02)00053-2.

Authors

Dongeun Yong¹, Rojin Park, Jong Hwa Yum, Kyungwon Lee, Eung Chil Choi, Yunsop Chong

Affiliation

¹ Department of Clinical Pathology and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea.

PMID: 12223302
DOI: 10.1016/s0167-7012(02)00053-2

Abstract

Cefoxitin-resistant Escherichia coli and Klebsiella pneumoniae are widespread in Korea. Significant proportions of them are considered to be CMY-1 producers. For effective screening of CMY-1 producers, the Hodge test was modified by using a cefoxitin disk and the performance was evaluated. The sensitivity and specifity of the test were 100% and 94.9%, respectively. The test was easier to perform than the three-dimensional extract test. This modified test should be suitable for screening CMY-1-producing strains of E. coli and K. pneumoniae.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology*
Bacterial Proteins*
Cefoxitin / pharmacology*
Drug Resistance, Bacterial
Escherichia coli / drug effects
Escherichia coli / enzymology
Escherichia coli / isolation & purification*
Humans
Klebsiella pneumoniae / drug effects
Klebsiella pneumoniae / enzymology
Klebsiella pneumoniae / isolation & purification*
Microbial Sensitivity Tests / instrumentation
Microbial Sensitivity Tests / methods
Sensitivity and Specificity
beta-Lactamases / metabolism*

Substances

Anti-Bacterial Agents
Bacterial Proteins
Cefoxitin
AmpC beta-lactamases
beta-Lactamases