Abstract
Galectin-9 is a member of the galectin family and has been identified as an eosinophil chemoattractant produced by activated T lymphocytes. Vascular endothelial cells play an important role in the initial step of eosinophil recruitment and activation in immune and inflammatory responses. We have addressed the stimulation of galectin-9 expression in endothelial cells. Galectin-9 was detected in membrane and cytosolic fractions of human umbilical vein endothelial cells stimulated with interferon-gamma (IFN-gamma). IFN-gamma also enhanced the adhesion of human eosinophilic leukemia-1 cells to endothelial monolayers, and it was inhibited by the presence of lactose. Interleukin-4, which induces eotaxin expression, did not affect the expression of galectin-9. The in situ endothelium from patients with inflammatory diseases was found to express galectin-9. IFN-gamma-induced production of galectin-9 by endothelial cells may play an important role in immune responses by regulating interactions between the vascular wall and eosinophils.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Autoimmune Diseases / metabolism
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Autoimmune Diseases / pathology
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Cell Adhesion / drug effects
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Cell Membrane / metabolism
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Cells, Cultured / drug effects
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Cells, Cultured / metabolism
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Chemokine CCL11
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Chemokines, CC / biosynthesis
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Chemokines, CC / genetics
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Cytosol / metabolism
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Endothelium, Vascular / drug effects*
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Endothelium, Vascular / metabolism
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Eosinophils / cytology
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Galectins*
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Gene Expression Regulation / drug effects*
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Humans
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Hypereosinophilic Syndrome / pathology
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Interferon-gamma / pharmacology*
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Interleukin-4 / pharmacology
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Lactose / pharmacology
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Lectins / biosynthesis*
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Lectins / genetics
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Nasal Polyps / chemistry
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Recombinant Proteins
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Rhinitis, Allergic, Seasonal / metabolism
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Rhinitis, Allergic, Seasonal / pathology
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Sjogren's Syndrome / metabolism
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Sjogren's Syndrome / pathology
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Tumor Cells, Cultured / cytology
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Tumor Cells, Cultured / drug effects
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Umbilical Veins
Substances
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CCL11 protein, human
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Chemokine CCL11
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Chemokines, CC
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Galectins
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LGALS9 protein, human
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Lectins
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Recombinant Proteins
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Interleukin-4
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Interferon-gamma
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Lactose