Tyrosine kinase-type receptor ErbB4 in chondrocytes: interaction with connective tissue growth factor and distribution in cartilage

Kumiko Nawachi; Miho Inoue; Satoshi Kubota; Takashi Nishida; Gen Yosimichi; Tohru Nakanishi; Manabu Kanyama; Takuo Kuboki; Hirofumi Yatani; Tomoichiro Yamaai; Masaharu Takigawa

doi:10.1016/s0014-5793(02)03263-5

Tyrosine kinase-type receptor ErbB4 in chondrocytes: interaction with connective tissue growth factor and distribution in cartilage

FEBS Lett. 2002 Sep 25;528(1-3):109-13. doi: 10.1016/s0014-5793(02)03263-5.

Authors

Kumiko Nawachi¹, Miho Inoue, Satoshi Kubota, Takashi Nishida, Gen Yosimichi, Tohru Nakanishi, Manabu Kanyama, Takuo Kuboki, Hirofumi Yatani, Tomoichiro Yamaai, Masaharu Takigawa

Affiliation

¹ Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine and Dentistry, Japan.

PMID: 12297288
DOI: 10.1016/s0014-5793(02)03263-5

Abstract

In order to identify receptor molecules that participate in the growth and differentiation of chondrocytes, we cloned a number of cDNA fragments from HCS-2/8 chondrocytic cells, by using tyrosine kinase-specific primers for amplification. The mRNA expression of one such receptor, ErbB4, was increased by connective tissue growth factor/hypertrophic chondrocyte-specific gene product (CTGF/Hcs24), which promotes all stages of the endochondral ossification in vitro. ErbB4 expression was observed through all stages of chondrocytic differentiation in vitro, corresponding to the wide distribution of CTGF/Hcs24 target cells. Furthermore, positive signals for erbB4 mRNA were detectable throughout most populations of chondrocytes, in growth and articular cartilage in vivo. These results demonstrate for the first time that ErbB4 is expressed in chondrocytes and may play some roles in chondrocytic growth and differentiation along with CTGF/Hcs24.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Cartilage / growth & development
Cartilage / metabolism*
Cartilage, Articular / growth & development
Cartilage, Articular / metabolism
Cell Differentiation
Cell Line
Chondrocytes / cytology
Chondrocytes / drug effects
Chondrocytes / metabolism*
Connective Tissue Growth Factor
ErbB Receptors / chemistry
ErbB Receptors / genetics
ErbB Receptors / metabolism*
Growth Plate / growth & development
Growth Plate / metabolism
Humans
Immediate-Early Proteins / genetics
Immediate-Early Proteins / metabolism*
Immediate-Early Proteins / pharmacology
Intercellular Signaling Peptides and Proteins / genetics
Intercellular Signaling Peptides and Proteins / metabolism*
Intercellular Signaling Peptides and Proteins / pharmacology
Mice
Mice, Inbred ICR
Molecular Sequence Data
Protein Structure, Tertiary
Receptor, ErbB-4
Recombinant Proteins / pharmacology
Sequence Homology, Amino Acid
Tissue Distribution
Up-Regulation / drug effects

Substances

CCN2 protein, human
CCN2 protein, mouse
Immediate-Early Proteins
Intercellular Signaling Peptides and Proteins
Recombinant Proteins
Connective Tissue Growth Factor
ERBB4 protein, human
ErbB Receptors
Erbb4 protein, mouse
Receptor, ErbB-4