Abstract
The ability of HIV-1 to use dendritic cells (DCs) for transport and to transfer virus to activated T cells in the lymph node may be crucial in early HIV-1 pathogenesis. We have characterized primary DCs for the receptors involved in viral envelope attachment and observed that C-type lectin receptor (CLR) binding was predominant in skin DCs, whereas binding to emigrating and tonsil DCs was CD4-dependent. No one CLR was solely responsible for envelope binding on all skin DC subsets. DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin) was only expressed by CD14(+)CDla(lo) dermal DCs. The mannose receptor was expressed by CD1a(hi) and CD14(+)CDla(lo) dermal DCs, and langerin was expressed by Langerhans cells. The diversity of CLRs able to bind HIV-1 in skin DCs may reflect their ability to bind a range of microbial glycoproteins.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antigens, CD
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Antigens, Surface / metabolism*
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CD4 Antigens / metabolism*
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CD4-Positive T-Lymphocytes / metabolism
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Cell Adhesion Molecules*
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Cell Line, Transformed
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Cells, Cultured
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Dendritic Cells / cytology
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Dendritic Cells / metabolism
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HIV Envelope Protein gp120 / metabolism*
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HIV-1 / metabolism*
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Humans
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Langerhans Cells / cytology
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Langerhans Cells / metabolism*
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Lectins / metabolism*
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Lectins, C-Type*
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Mannose Receptor
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Mannose-Binding Lectins*
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Receptors, Cell Surface / metabolism*
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Receptors, HIV / metabolism*
Substances
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Antigens, CD
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Antigens, Surface
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CD207 protein, human
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CD4 Antigens
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Cell Adhesion Molecules
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DC-specific ICAM-3 grabbing nonintegrin
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HIV Envelope Protein gp120
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Lectins
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Lectins, C-Type
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Mannose Receptor
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Mannose-Binding Lectins
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Receptors, Cell Surface
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Receptors, HIV